Identification and characterization of an R-Smad homologue (Hco-DAF-8) from Haemonchus contortus.

ABSTRACT:

Background

Smad proteins are essential cellular mediators within the transforming growth factor-β (TGF-β) superfamily. They directly transmit incoming signals from the cell surface receptors to the nucleus. In spite of their functional importance, almost nothing is known about Smad proteins in parasitic nematodes including Haemonchus contortus, an important blood-sucking nematode of small ruminants.

Methods

Based on genomic and transcriptome data for H. contortus and using bioinformatics methods, a Smad homologue (called Hco-daf-8) was inferred from H. contortus and the structural characteristics of this gene and its encoded protein Hco-DAF-8 established. Using real-time PCR and immunofluorescence assays, temporal transcriptional and spatial expression profiles of Hco-daf-8

SUBMITTER: Li FF 

PROVIDER: S-EPMC7119156 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature