Detection of Bovine Coronavirus by Conventional Reverse Transcription Polymerase Chain Reaction

ABSTRACT: Bovine coronavirus (BCoV) is an economically significant cause of enteric and respiratory diseases in cattle throughout the world. BCoV is a known cause of neonatal calf diarrhea, winter dysentery in adult cattle, and respiratory disorders in cattle of all ages. In this chapter, we describe a simple and efficient protocol for total nucleic acids extraction to be used in conventional RT-PCR assay. This is a technique used routinely in our virology laboratory to detect BCoV from stool and nasopharyngeal samples of cattle.

SUBMITTER: Wang L 

PROVIDER: S-EPMC7121124 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature