A comparison of human serum and plasma metabolites using untargeted ¹H NMR spectroscopy and UPLC-MS.

ABSTRACT:

Introduction

Differences in the metabolite profiles between serum and plasma are incompletely understood.

Objectives

To evaluate metabolic profile differences between serum and plasma and among plasma sample subtypes.

Methods

We analyzed serum, platelet rich plasma (PRP), platelet poor plasma (PPP), and platelet free plasma (PFP), collected from 8 non-fasting apparently healthy women, using untargeted standard 1D and CPMG ¹H NMR and reverse phase and hydrophilic (HILIC) UPLC-MS. Differences between metabolic profiles were evaluated using validated principal component and orthogonal partial least squares discriminant analysis.

Results

Explorative analysis showed the main source of variation among samples was due to inter-individual differences with no

SUBMITTER: Kaluarachchi M 

PROVIDER: S-EPMC7122646 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature