Deletion of MHY1 abolishes hyphae formation in Yarrowia lipolytica without negative effects on stress tolerance.

ABSTRACT: There is a need for development of sustainable production processes for production of fats/oils and lipid derived chemicals. The dimorphic oleaginous yeast Yarrowia lipolytica is a promising organism for conversion of biomass hydrolysate to lipids, but in many such processes hyphae formation will be problematic. We have therefore constructed and compared the performance of strains carrying deletions in several published gene targets suggested to abolish hyphae formation (MHY1, HOY1 and CLA4). The MHY1-deletion was the only of the tested strains which did not exhibit hyphae formation under any of the conditions tested. The MHY1-deletion also had a weak positive effect on lipid accumulation without affecting the total fatty acid composition, irrespective of the nitrogen source used. MHY1 has

SUBMITTER: Konzock O 

PROVIDER: S-EPMC7122783 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature