Cell Cycle Profiling Reveals Protein Oscillation, Phosphorylation, and Localization Dynamics.

ABSTRACT: The cell cycle is a highly conserved process involving the coordinated separation of a single cell into two daughter cells. To relate transcriptional regulation across the cell cycle with oscillatory changes in protein abundance and activity, we carried out a proteome- and phospho-proteome-wide mass spectrometry profiling. We compared protein dynamics with gene transcription, revealing many transcriptionally regulated G2 mRNAs that only produce a protein shift after mitosis. Integration of CRISPR/Cas9 survivability studies further highlighted proteins essential for cell viability. Analyzing the dynamics of phosphorylation events and protein solubility dynamics over the cell cycle, we characterize predicted phospho-peptide motif distributions and predict cell cycle-dependent translocating p

SUBMITTER: Herr P 

PROVIDER: S-EPMC7124475 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature