First documentation of major Vip3Aa resistance alleles in field populations of Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) in Texas, USA.

ABSTRACT: The corn earworm, Helicoverpa zea, is a major target pest of the insecticidal Vip3Aa protein used in pyramided transgenic Bt corn and cotton with Cry1 and Cry2 proteins in the U.S. The widespread resistance to Cry1 and Cry2 proteins in H. zea will challenge the long-term efficacy of Vip3Aa technology. Determining the frequency of resistant alleles to Vip3Aa in field populations of H. zea is critically important for resistance management. Here, we provided the first F₂ screen study to estimate the resistance allele frequency for Vip3Aa in H. zea populations in Texas, U.S. In 2019, 128 H. zea neonates per isofamily for a total of 114 F₂ families were screened with a diagnostic concentration of 3.0 μg/cm² of Vip3Aa39 protein in diet-overlay bioassays. The F

SUBMITTER: Yang F 

PROVIDER: S-EPMC7125131 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature