Translational derepression of Elavl4 isoforms at their alternative 5' UTRs determines neuronal development.

ABSTRACT: Neurodevelopment requires precise regulation of gene expression, including post-transcriptional regulatory events such as alternative splicing and mRNA translation. However, translational regulation of specific isoforms during neurodevelopment and the mechanisms behind it remain unknown. Using RNA-seq analysis of mouse neocortical polysomes, here we report translationally repressed and derepressed mRNA isoforms during neocortical neurogenesis whose orthologs include risk genes for neurodevelopmental disorders. We demonstrate that the translation of distinct mRNA isoforms of the RNA binding protein (RBP), Elavl4, in radial glia progenitors and early neurons depends on its alternative 5' UTRs. Furthermore, 5' UTR-driven Elavl4 isoform-specific translation depends on upstream control by anoth

SUBMITTER: Popovitchenko T 

PROVIDER: S-EPMC7125149 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature