Streamlined selection of cancer antigens for vaccine development through integrative multi-omics and high-content cell imaging.

ABSTRACT: Identification of tumor antigens that induce cytotoxic T lymphocytes (CTLs) is crucial for cancer-vaccine development. Despite their predictive ability, current algorithmic approaches and human leukocyte antigen (HLA)-peptidomic analysis allow limited selectivity. Here, we optimized a method to rapidly screen and identify highly immunogenic epitopes that trigger CTL responses. We used a combined application of this method involving immune-specific signature analysis and HLA-associated peptidomics using samples from six patients with triple-negative breast cancer (TNBC) in order to select immunogenic HLA epitopes for in vitro testing. Additionally, we applied high-throughput imaging at the single-cell level in order to confirm the immunoreactivity of the selected peptides. The results indic

SUBMITTER: Han KC 

PROVIDER: S-EPMC7125174 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature