Ontology highlight
ABSTRACT:
SUBMITTER: Dao Thi VL
PROVIDER: S-EPMC7125181 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Nature communications 20200403 1
Human stem cell-derived hepatocyte-like cells (HLCs) offer an attractive platform to study liver biology. Despite their numerous advantages, HLCs lack critical in vivo characteristics, including cell polarity. Here, we report a stem cell differentiation protocol that uses transwell filters to generate columnar polarized HLCs with clearly defined basolateral and apical membranes separated by tight junctions. We show that polarized HLCs secrete cargo directionally: Albumin, urea, and lipoproteins ...[more]