Temporal Dynamics and Heterogeneity of Cell Populations during Skeletal Muscle Regeneration.

ABSTRACT: Mammalian skeletal muscle possesses a unique ability to regenerate, which is primarily mediated by a population of resident muscle stem cells (MuSCs) and requires a concerted response from other supporting cell populations. Previous targeted analysis has described the involvement of various specific populations in regeneration, but an unbiased and simultaneous evaluation of all cell populations has been limited. Therefore, we used single-cell RNA-sequencing to uncover gene expression signatures of over 53,000 individual cells during skeletal muscle regeneration. Cells clustered into 25 populations and subpopulations, including a subpopulation of immune gene enriched myoblasts (immunomyoblasts) and subpopulations of fibro-adipogenic progenitors. Our analyses also uncovered striking spatiote

SUBMITTER: Oprescu SN 

PROVIDER: S-EPMC7125354 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature