Down-regulation of transcription of the proapoptotic gene BNip3 in cultured astrocytes by murine coronavirus infection.

ABSTRACT: Murine coronavirus mouse hepatitis virus (MHV) causes encephalitis and demyelination in the central nervous system of susceptible rodents. Astrocytes are the major target for MHV persistence. However, the mechanisms by which astrocytes survive MHV infection and permit viral persistence are not known. Here we performed DNA microarray analysis on differential gene expression in astrocyte DBT cells by MHV infection and found that the mRNA of the proapoptotic gene BNip3 was significantly decreased following MHV infection. This finding was further confirmed by quantitative reverse transcription-polymerase chain reaction, Western blot analysis, and BNip3-promoter-luciferase reporter system. Interestingly, infection with live and ultraviolet light-inactivated viruses equally repressed BNip3 expre

SUBMITTER: Cai Y 

PROVIDER: S-EPMC7125541 | biostudies-literature | 2003 Nov

REPOSITORIES: biostudies-literature