Electrochemical nucleic acid detection based on parallel structural dsDNA/recombinant azurin hybrid.

ABSTRACT: Several challenges remained to fabricate a molecular-level nucleic acid biosensor such as surface immobilization control, single mismatch detection and low current response. To overcome those issues, for the first time, authors presented a novel parallel structural dsDNA/recombinant azurin (PSD/rAzu) hybrid structure for the general nucleic acid detection. The PSD was designed and introduced by the optimized 8 Ag⁺ ions to have greater conductivity than the canonical dsDNA, and conjugated with rAzu to develop a general platform for electrochemical detection of miRNAs and viral DNAs with high reproducibility and ultra-sensitivity towards single base pair mutation. Thanks to the bifunctional rAzu as the selective spacer and electrochemical signal mediator, in the presence of the ta

SUBMITTER: Mohammadniaei M 

PROVIDER: S-EPMC7125781 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature