Isolation and characterization of a novel mesonivirus from Culex mosquitoes in China.

ABSTRACT: A new insect nidovirus (named Yichang virus) from the family Mesoniviridae was isolated, identified, and characterized from Culex mosquitoes in Hubei, China. Results showed a high number of viral RNA copies (up to 10¹¹ copies/ml) within 48h in C6/36 cells. In addition, the titers of the Yichang virus reached maximal levels of 10⁷ PFU/mL at 6 d post-infection (dpi). The virus produced moderate cytopathic effects when the multiplicity of infection ranged from 0.001-0.1 at 6 dpi, but did not replicate in mammalian cells. Under electron microscopy, the virion of the Yichang virus appeared as spherical particles with diameters of ∼80nm and large club-shaped projections. Although subsequent genomic sequence analysis revealed that the Yichang virus had similar protein patter

SUBMITTER: Wang Y 

PROVIDER: S-EPMC7125798 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature