Challenges and opportunities in the purification of recombinant tagged proteins.

ABSTRACT: The purification of recombinant proteins by affinity chromatography is one of the most efficient strategies due to the high recovery yields and purity achieved. However, this is dependent on the availability of specific affinity adsorbents for each particular target protein. The diversity of proteins to be purified augments the complexity and number of specific affinity adsorbents needed, and therefore generic platforms for the purification of recombinant proteins are appealing strategies. This justifies why genetically encoded affinity tags became so popular for recombinant protein purification, as these systems only require specific ligands for the capture of the fusion protein through a pre-defined affinity tag tail. There is a wide range of available affinity pairs "tag-ligand" combini

SUBMITTER: Pina AS 

PROVIDER: S-EPMC7125906 | biostudies-literature | 2014 Mar-Apr

REPOSITORIES: biostudies-literature