Isotopic and genetic methods reveal the role of the gut microbiome in mammalian host essential amino acid metabolism.

ABSTRACT: Intestinal microbiota perform many functions for their host, but among the most important is their role in metabolism, especially the conversion of recalcitrant biomass that the host is unable to digest into bioavailable compounds. Most studies have focused on the assistance gut microbiota provide in the metabolism of carbohydrates, however, their role in host amino acid metabolism is poorly understood. We conducted an experiment on Mus musculus using 16S rRNA gene sequencing and carbon isotope analysis of essential amino acids (AA_ESS) to quantify the community composition of gut microbiota and the contribution of carbohydrate carbon used by the gut microbiome to synthesize AA_ESS that are assimilated by mice to build skeletal muscle tissue. The relative abundan

SUBMITTER: Newsome SD 

PROVIDER: S-EPMC7126075 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature