Modeling of shotgun sequencing of DNA plasmids using experimental and theoretical approaches.

ABSTRACT:

Background

Processing and analysis of DNA sequences obtained from next-generation sequencing (NGS) face some difficulties in terms of the correct prediction of DNA sequencing outcomes without the implementation of bioinformatics approaches. However, algorithms based on NGS perform inefficiently due to the generation of long DNA fragments, the difficulty of assembling them and the complexity of the used genomes. On the other hand, the Sanger DNA sequencing method is still considered to be the most reliable; it is a reliable choice for virtual modeling to build all possible consensus sequences from smaller DNA fragments.

Results

In silico and in vitro experiments were conducted: (1) to implement and test our novel sequencing algorithm, using the standard cloning vectors of dif

SUBMITTER: Shityakov S 

PROVIDER: S-EPMC7126183 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature