Isolation and characterization of a variant subgroup GII-a porcine epidemic diarrhea virus strain in China.

ABSTRACT:

Background

Highly virulent variants of porcine epidemic diarrhea virus (PEDV) have been closely associated with recent outbreaks of porcine epidemic diarrhea (PED) in China, which have resulted in severe economic losses to the pork industry.

Methods

In the current study, the variant PEDV strain HM2017 was isolated and purified and a viral growth curve was constructed according to the median tissue culture infective dose (TCID50). HM2017 were amplify with RT-PCR and analyzed by phylogeny analysis. Animal pathogenicity experiment was carried to evaluate the HM2017 clinical assessment.

Results

Genome-based phylogenetic analysis revealed that PEDV strain HM2017 was clustered into the variant subgroup GII-a that is currently circulating in pig populations in China. The hig

SUBMITTER: Yang D 

PROVIDER: S-EPMC7126716 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature