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Real-time reverse transcriptase polymerase chain reaction assays for Middle East Respiratory Syndrome.

ABSTRACT: Here we designed and tested two highly specific quantitative TaqMan(®)-MGB-based reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) assays for Middle East Respiratory Syndrome (MERS). The primers and probes for these assays were evaluated and found to have a limit of detection (LOD) of 0.005 plaque-forming units/PCR (pfu/PCR).

SUBMITTER: Douglas CE 

PROVIDER: S-EPMC7126732 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature

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Real-time reverse transcriptase polymerase chain reaction assays for Middle East Respiratory Syndrome.

Douglas Christina E CE   Kulesh David A DA   Jaissle James G JG   Minogue Timothy D TD  

Molecular and cellular probes 20150911 6

Here we designed and tested two highly specific quantitative TaqMan(®)-MGB-based reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) assays for Middle East Respiratory Syndrome (MERS). The primers and probes for these assays were evaluated and found to have a limit of detection (LOD) of 0.005 plaque-forming units/PCR (pfu/PCR). ...[more]

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