Upregulation of the RNF8 gene can predict the presence of sperm in azoospermic individuals.

ABSTRACT: OBJECTIVE:In this study, specimens from testicular biopsies of men with nonobstructive azoospermia (NOA) were used to investigate whether RNF8 gene could serve as a biomarker to predict the presence of sperm in these patients. METHODS:Testicular biopsy specimens from 47 patients were classified according to the presence of sperm (positive vs. negative groups) and investigated for the expression of RNF8. The level of RNF8 gene expression in the testes was compared between these groups using reverse-transcription polymerase chain reaction. RESULTS:The expression level of RNF8 was significantly higher in testicular samples from the positive group than in those from the negative group. Moreover, the area under the curve of RNF8 expression for the entire study population was 0.84, showing the d

SUBMITTER: Nazari M 

PROVIDER: S-EPMC7127899 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature