A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses.

ABSTRACT:

Objective

The re-emergence and spread of tropical viruses to new areas has raised a wave of concern worldwide. In order to treat patients at an early stage and prevent the diffusion of an outbreak, early diagnosis, and therefore fast and adequate detection, is needed. To this end, a multiplex reverse transcription real-time polymerase chain reaction TaqMan method was designed to detect Zika (ZIKV) and chikungunya (CHIKV) viruses simultaneously.

Methods

Two methods targeting different genome segments were selected from the literature for each virus. These were adapted for high genome coverage and combined in a four-plex assay that was thoroughly validated in-house. The SCREENED tool was used to evaluate the sequence coverage of the method.

Results

The full validation a

SUBMITTER: Broeders S 

PROVIDER: S-EPMC7129992 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature