Full-length transcriptome sequencing provides insights into the evolution of apocarotenoid biosynthesis in Crocus sativus.

ABSTRACT: Crocus sativus, containing remarkably amounts of crocin, picrocrocin and safranal, is the source of saffron with tremendous medicinal, economic and cultural importance. Here, we present a high-quality full-length transcriptome of the sterile triploid C. sativus, using the PacBio SMRT sequencing technology. This yields 31,755 high-confidence predictions of protein-coding genes, with 50.1% forming paralogous gene pairs. Analysis on distribution of Ks values suggests that the current genome of C. sativus is probably a product resulting from at least two rounds of whole-genome duplication (WGD) events occurred at ~28 and ~114 million years ago (Mya), respectively. We provide evidence demonstrating that the recent β WGD event confers a major impact on family expansio

SUBMITTER: Yue J 

PROVIDER: S-EPMC7132054 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature