Timing and original features of flagellum assembly in trypanosomes during development in the tsetse fly.

ABSTRACT: BACKGROUND:Trypanosoma brucei exhibits a complex life-cycle alternating between tsetse flies and mammalian hosts. When parasites infect the fly, cells differentiate to adapt to life in various tissues, which is accompanied by drastic morphological and biochemical modifications especially in the proventriculus. This key step represents a bottleneck for salivary gland infection. METHODS:Here, we monitored flagellum assembly in trypanosomes during differentiation from the trypomastigote to the epimastigote stage, i.e. when the nucleus migrates to the posterior end of the cell, by using three-dimensional electron microscopy (focused ion beam scanning electron microscopy, FIB-SEM) and immunofluorescence assays. RESULTS:The combination of light and electron microscopy approaches provided structu

SUBMITTER: Lemos M 

PROVIDER: S-EPMC7132888 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature