TFPI2 suppresses breast cancer progression through inhibiting TWIST-integrin α5 pathway.

ABSTRACT:

Background

Tissue factor pathway inhibitor 2 (TFPI2) participates in carcinogenesis of various tumors, and is associated with poor survival of breast cancer patients. However, the effect and underlying mechanism of TFPI2 on breast cancer progression remains to be investigated.

Methods

The expression level of TFPI2 in breast cancer tissues and cell lines was examined via qRT-PCR (quantitative real-time polymerase chain reaction) and immunohistochemistry. CCK8 (Cell Counting Kit-8), colony formation, wound healing or transwell assays were used to detect cell viability, proliferation, migration or invasion, respectively. In vivo subcutaneous xenotransplanted tumor model was established to detect tumorigenic function of TFPI2, and the underlying mechanism was evaluated by immuno

SUBMITTER: Zhao D 

PROVIDER: S-EPMC7133004 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature