Absolute measurement of the tissue origins of cell-free DNA in the healthy state and following paracetamol overdose.

ABSTRACT:

Background

Despite the emergence of cell-free DNA (cfDNA) as a clinical biomarker in cancer, the tissue origins of cfDNA in healthy individuals have to date been inferred only by indirect and relative measurement methods, such as tissue-specific methylation and nucleosomal profiling.

Methods

We performed the first direct, absolute measurement of the tissue origins of cfDNA, using tissue-specific knockout mouse strains, in both healthy mice and following paracetamol (APAP) overdose. We then investigated the utility of total cfDNA and the percentage of liver-specific cfDNA as clinical biomarkers in patients presenting with APAP overdose.

Results

Analysis of cfDNA from healthy tissue-specific knockout mice showed that cfDNA originates predominantly from white and red blo

SUBMITTER: Laurent D 

PROVIDER: S-EPMC7133021 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature