Cardiac Myosin Promotes Thrombin Generation and Coagulation In Vitro and In Vivo.

ABSTRACT:

Objective

Cardiac myosin (CM) is structurally similar to skeletal muscle myosin, which has procoagulant activity. Here, we evaluated CM's ex vivo, in vivo, and in vitro activities related to hemostasis and thrombosis. Approach and Results: Perfusion of fresh human blood over CM-coated surfaces caused thrombus formation and fibrin deposition. Addition of CM to blood passing over collagen-coated surfaces enhanced fibrin formation. In a murine ischemia/reperfusion injury model, exogenous CM, when administered intravenously, augmented myocardial infarction and troponin I release. In hemophilia A mice, intravenously administered CM reduced tail-cut-initiated bleeding. These data provide proof of concept for CM's in vivo procoagulant properties. In vitro studies clarified some mechanisms

SUBMITTER: Zilberman-Rudenko J 

PROVIDER: S-EPMC7135739 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature