Enrichment of rare events using a multi-parameter high throughput microfluidic droplet sorter.

ABSTRACT: High information content analysis, enrichment, and selection of rare events from a large population are of great importance in biological and biomedical research. The fluorescence lifetime of a fluorophore, a photophysical property which is independent of and complementary to fluorescence intensity, has been incorporated into various imaging and sensing techniques through microscopy, flow cytometry and droplet microfluidics. However, the throughput of fluorescence lifetime activated droplet sorting is orders of magnitude lower than that of fluorescence activated cell sorting, making it unattractive for applications such as directed evolution of enzymes, despite its highly effective compartmentalization of library members. We developed a microfluidic sorter capable of selecting fluorophores

SUBMITTER: Hung ST 

PROVIDER: S-EPMC7135947 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature