Mammalian lectin arrays for screening host-microbe interactions.

ABSTRACT: Many members of the C-type lectin family of glycan-binding receptors have been ascribed roles in the recognition of microorganisms and serve as key receptors in the innate immune response to pathogens. Other mammalian receptors have become targets through which pathogens enter target cells. These receptor roles have often been documented with binding studies involving individual pairs of receptors and microorganisms. To provide a systematic overview of interactions between microbes and the large complement of C-type lectins, here we developed a lectin array and suitable protocols for labeling of microbes that could be used to probe this array. The array contains C-type lectins from cow, chosen as a model organism of agricultural interest for which the relevant pathogen-receptor interaction

SUBMITTER: Jegouzo SAF 

PROVIDER: S-EPMC7135977 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature