Mechanical stretch increases Kv1.5 current through an interaction between the S1-S2 linker and N-terminus of the channel.

ABSTRACT: The voltage-gated potassium channel Kv1.5 plays important roles in atrial repolarization and regulation of vascular tone. In the present study, we investigated the effects of mechanical stretch on Kv1.5 channels. We induced mechanical stretch by centrifuging or culturing Kv1.5-expressing HEK 293 cells and neonatal rat ventricular myocytes in low osmolarity (LO) medium and then recorded Kv1.5 current (I_Kv1.5) in a normal, isotonic solution. We observed that mechanical stretch increased I_Kv1.5, and this increase required the intact, long, proline-rich extracellular S1-S2 linker of the Kv1.5 channel. The low osmolarity-induced I_Kv1.5 increase also required an intact intracellular N terminus, which contains the binding motif for endogenous Src tyrosine kinase t

SUBMITTER: Milton AO 

PROVIDER: S-EPMC7136002 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature