Organoid cultures from normal and cancer-prone human breast tissues preserve complex epithelial lineages.

ABSTRACT: Recently, organoid technology has been used to generate a large repository of breast cancer organoids. Here we present an extensive evaluation of the ability of organoid culture technology to preserve complex stem/progenitor and differentiated cell types via long-term propagation of normal human mammary tissues. Basal/stem and luminal progenitor cells can differentiate in culture to generate mature basal and luminal cell types, including ER+ cells that have been challenging to maintain in culture. Cells associated with increased cancer risk can also be propagated. Single-cell analyses of matched organoid cultures and native tissues by mass cytometry for 38 markers provide a higher resolution representation of the multiple mammary epithelial cell types in the organoids, and demonstrate that

SUBMITTER: Rosenbluth JM 

PROVIDER: S-EPMC7136203 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature