Toward a genome scale sequence specific dynamic model of cell-free protein synthesis in Escherichia coli.

ABSTRACT: In this study, we developed a dynamic mathematical model of E. coli cell-free protein synthesis (CFPS). Model parameters were estimated from a dataset consisting of glucose, organic acids, energy species, amino acids, and protein product, chloramphenicol acetyltransferase (CAT) measurements. The model was successfully trained to simulate these measurements, especially those of the central carbon metabolism. We then used the trained model to evaluate the performance, e.g., the yield and rates of protein production. CAT was produced with an energy efficiency of 12%, suggesting that the process could be further optimized. Reaction group knockouts showed that protein productivity was most sensitive to the oxidative phosphorylation and glycolysis/gluconeogenesis pathways. Amino acid biosynthesi

SUBMITTER: Horvath N 

PROVIDER: S-EPMC7136494 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature