Assessment of the Role of C3(H₂O) in the Alternative Pathway.

ABSTRACT: In this study we investigate the hydrolysis of C3 to C3(H₂O) and its ability to initiate activation via the alternative pathway (AP) of the complement system. The internal thioester bond within C3 is hydrolyzed by water in plasma because of its inherent lability. This results in the formation of non-proteolytically activated C3(H₂O) which is believed have C3b-like properties and be able to form an active initial fluid phase C3 convertase together with Factor B (FB). The generation of C3(H₂O) occurs at a low but constant rate in blood, but the formation can be greatly accelerated by the interaction with various surfaces or nucleophilic and chaotropic agents. In order to more specifically elucidate the relevance of the C3(H₂O) for AP activation, fo

SUBMITTER: Fromell K 

PROVIDER: S-EPMC7136553 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature