Coenzyme Coupling Boosts Charge Transport through Single Bioactive Enzyme Junctions.

ABSTRACT: Oxidation of formate to CO₂ is catalyzed via the donation of electrons from formate dehydrogenase (FDH) to nicotinamide adenine dinucleotide (NAD⁺), and thus the charge transport characteristics of FDH become essential but remain unexplored. Here, we investigated the charge transport through single-enzyme junctions of FDH using the scanning tunneling microscope break junction technique (STM-BJ). We found that the coupling of NAD⁺ with FDH boosts the charge transport by ∼2,100%, and the single-enzyme conductance highly correlates with the enzyme activity. The combined flicker noise analysis demonstrated the switching of the coenzyme-mediated charge transport pathway and supported by the significantly reduced HOMO-LUMO gap from calculations. Site-specific mut

SUBMITTER: Zhuang X 

PROVIDER: S-EPMC7136626 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature