Multilevel optimisation of anaerobic ethyl acetate production in engineered Escherichia coli.

ABSTRACT:

Background

Ethyl acetate is a widely used industrial solvent that is currently produced by chemical conversions from fossil resources. Several yeast species are able to convert sugars to ethyl acetate under aerobic conditions. However, performing ethyl acetate synthesis anaerobically may result in enhanced production efficiency, making the process economically more viable.

Results

We engineered an E. coli strain that is able to convert glucose to ethyl acetate as the main fermentation product under anaerobic conditions. The key enzyme of the pathway is an alcohol acetyltransferase (AAT) that catalyses the formation of ethyl acetate from acetyl-CoA and ethanol. To select a suitable AAT, the ethyl acetate-forming capacities of Atf1 from Saccharomyces cerevisiae,

SUBMITTER: Bohnenkamp AC 

PROVIDER: S-EPMC7137189 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature