Preparation of uniformly labelled ¹³C- and ¹⁵N-plants using customised growth chambers.

ABSTRACT:

Background

Stable isotopically labelled organisms have found wide application in life science research including plant physiology, plant stress and defense as well as metabolism related sciences. Therefore, the reproducible production of plant material enriched with stable isotopes such as ¹³C and ¹⁵N is of considerable interest. A high degree of enrichment (> 96 atom %) with a uniformly distributed isotope (global labelling) is accomplished by a continuous substrate supply during plant growth/cultivation. In the case of plants, ¹³C-labelling can be achieved by growth in ¹³CO_2(g) atmosphere while global ¹⁵N-labelling needs ¹⁵N- containing salts in the watering/nutrient solution. Here, we present a method f

SUBMITTER: Ceranic A 

PROVIDER: S-EPMC7137243 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature