In-silico detection of aneuploidy and chromosomal deletions in wheat using genotyping-by-sequencing.

ABSTRACT:

Background

Short read sequencing technologies, such as genotyping-by-sequencing (GBS), have been utilized in genetic mapping, marker development, and population genomic studies. High-throughput and multiplexing capability coupled with low cost make GBS an appropriate tool for molecular research. Here, we present the application of GBS to characterize wheat aneuploid stocks and detect chromosomal aberrations including aneuploidy and chromosomal deletions. These aneuploids are an important resource that have been used in wheat genetics and genomics studies to localize genes, determine physical positions, and develop chromosome bin maps.

Results

Using GBS, we mapped sequence reads and quantified read coverage across chromosome bins. Using this approach, we confirmed known delet

SUBMITTER: Singh N 

PROVIDER: S-EPMC7137276 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature