Identification of surface proteins in a clinical Staphylococcus haemolyticus isolate by bacterial surface shaving.

ABSTRACT:

Background

The skin commensal Staphylococcus haemolyticus is an emerging nosocomial pathogen. Despite its clinical relevance, published information about S. haemolyticus virulence factors is scarce. In this study, the adhesive and biofilm forming properties of ten clinical and ten commensal S. haemolyticus strains were examined using standard adhesion and biofilm assays. One of the clinical strains was used to identify expressed surface proteins using bacterial surface shaving. Protein abundance was examined by a comparative analysis between bacterial protein expression after human keratinocyte (HaCaT) colonization and growth in cell culture media supplemented with serum. Relative protein quantification was performed by labeling peptides with tandem mass tags (TMT) prior to Mass Sp

SUBMITTER: Wolden R 

PROVIDER: S-EPMC7137321 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature