Principles of RNA processing from analysis of enhanced CLIP maps for 150 RNA binding proteins.

ABSTRACT:

Background

A critical step in uncovering rules of RNA processing is to study the in vivo regulatory networks of RNA binding proteins (RBPs). Crosslinking and immunoprecipitation (CLIP) methods enable mapping RBP targets transcriptome-wide, but methodological differences present challenges to large-scale analysis across datasets. The development of enhanced CLIP (eCLIP) enabled the mapping of targets for 150 RBPs in K562 and HepG2, creating a unique resource of RBP interactomes profiled with a standardized methodology in the same cell types.

Results

Our analysis of 223 eCLIP datasets reveals a range of binding modalities, including highly resolved positioning around splicing signals and mRNA untranslated regions that associate with distinct RBP functions. Quantification of en

SUBMITTER: Van Nostrand EL 

PROVIDER: S-EPMC7137325 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature