The DNA hypermethylation phenotype of colorectal cancer liver metastases resembles that of the primary colorectal cancers.

ABSTRACT:

Background

Identifying molecular differences between primary and metastatic colorectal cancers-now possible with the aid of omics technologies-can improve our understanding of the biological mechanisms of cancer progression and facilitate the discovery of novel treatments for late-stage cancer. We compared the DNA methylomes of primary colorectal cancers (CRCs) and CRC metastases to the liver. Laser microdissection was used to obtain epithelial tissue (10 to 25 × 10⁶ μm²) from sections of fresh-frozen samples of primary CRCs (n = 6), CRC liver metastases (n = 12), and normal colon mucosa (n = 3). DNA extracted from tissues was enriched for methylated sequences with a methylCpG binding domain (MBD) polypeptide-based protocol and subjected to deep sequencing. Th

SUBMITTER: Orjuela S 

PROVIDER: S-EPMC7137338 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature