Adipose stem cells from patients with Crohn's disease show a distinctive DNA methylation pattern.

ABSTRACT:

Background

Crohn's disease (CD) is characterized by persistent inflammation and ulceration of the small or large bowel, and expansion of mesenteric adipose tissue, termed creeping fat (CF). We previously demonstrated that human adipose-derived stem cells (hASCs) from CF of patients with CD exhibit dysfunctional phenotypes, including a pro-inflammatory profile, high phagocytic capacity, and weak immunosuppressive properties. Importantly, these phenotypes persist in patients in remission and are found in all adipose depots explored including subcutaneous fat. We hypothesized that changes in hASCs are a consequence of epigenetic modifications.

Methods

We applied epigenome-wide profiling with a methylation array (Illumina EPIC/850k array) and gene expression analysis to explore

SUBMITTER: Serena C 

PROVIDER: S-EPMC7137346 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature