MiR-423-5p may regulate ovarian response to ovulation induction via CSF1.

ABSTRACT:

Background

We have previously shown that hsa-miR-423-5p expression in ovarian granulosa cells is decreased in high ovarian response populations. The objective of the present study was to find the target gene and mechanism for miR-423-5p involved in ovarian response regulation.

Methods

(a) TargetScan was used to predict the target gene of hsa-miR-423-5p. (b) A model for hsa-miR-423-5p overexpression or inhibition was constructed by transfecting KGN cells with lentivirus. CSF1 mRNA and protein expression and luciferase activity were measured. (c) The cell cycles of control and lentivirus treated KGN cells were analyzed. Western blot was used to measure the expression of CDKN1A in KGN cells. (d) The concentration of E₂ in KGN cell culture medium were measured.

Res

SUBMITTER: Xie S 

PROVIDER: S-EPMC7137414 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature