Differential CpG DNA methylation in peripheral naive CD4⁺ T-cells in early rheumatoid arthritis patients.

ABSTRACT:

Background

The genetic risk associated with rheumatoid arthritis (RA) includes genes regulating DNA methylation, one of the hallmarks of epigenetic re-programing, as well as many T-cell genes, with a strong MHC association, pointing to immunogenetic mechanisms as disease triggers leading to chronicity. The aim of our study was to explore DNA methylation in early, drug-naïve RA patients, towards a better understanding of early events in pathogenesis.

Result

Monocytes, naïve and memory CD4⁺ T-cells were sorted from 6 healthy controls and 10 RA patients. DNA methylation was assessed using a genome-wide Illumina 450K CpG promoter array. Differential methylation was confirmed using bisulfite sequencing for a specific gene promoter, ELISA for several cytokines and flow

SUBMITTER: Pitaksalee R 

PROVIDER: S-EPMC7137446 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature