Characterization of Porphyromonas gingivalis sialidase and disruption of its role in host-pathogen interactions.

ABSTRACT: Key to onset and progression of periodontitis is a complex relationship between oral bacteria and the host. The organisms most associated with severe periodontitis are the periodontal pathogens of the red complex: Tannerella forsythia, Treponema denticola and Porphyromonas gingivalis. These organisms express sialidases, which cleave sialic acid from host glycoproteins, and contribute to disease through various mechanisms. Here, we expressed and purified recombinant P. gingivalis sialidase SiaPG (PG_0352) and characterized its activity on a number of substrates, including host sialoglycoproteins and highlighting the inability to cleave diacetylated sialic acids - a phenomenon overcome by the NanS sialate-esterase from T. forsythia. Indeed SiaPG required Na

SUBMITTER: Frey AM 

PROVIDER: S-EPMC7137779 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature