Engineering the PduT shell protein to modify the permeability of the 1,2-propanediol microcompartment of Salmonella.

ABSTRACT: Bacterial microcompartments (MCPs) are protein-based organelles that consist of metabolic enzymes encapsulated within a protein shell. The function of MCPs is to optimize metabolic pathways by increasing reaction rates and sequestering toxic pathway intermediates. A substantial amount of effort has been directed toward engineering synthetic MCPs as intracellular nanoreactors for the improved production of renewable chemicals. A key challenge in this area is engineering protein shells that allow the entry of desired substrates. In this study, we used site-directed mutagenesis of the PduT shell protein to remove its central iron-sulfur cluster and create openings (pores) in the shell of the Pdu MCP that have varied chemical properties. Subsequently, in vivo and in vitro studies

SUBMITTER: Chowdhury C 

PROVIDER: S-EPMC7137781 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature