Single Hepatocyte Hepatitis B Virus Transcriptional Landscape in HIV Coinfection.

ABSTRACT:

Background

Hepatitis B virus (HBV) is a leading cause of liver failure and hepatocellular carcinoma. Approximately 10% of people with HIV also have HBV and are at higher risk of liver disease progression than in HBV monoinfection. Antivirals, common to HIV and HBV, suppress HBV DNA levels but do not eradicate virus because the transcriptional template, covalently closed circular DNA (cccDNA), is long lived in infected hepatocytes.

Methods

Using single-cell laser capture microdissection, we isolated >1100 hepatocytes from 5 HIV/HBV coinfected persons with increasing exposure to HBV antivirals (HB1-HB5; no exposure to >7 years exposure), quantifying cccDNA and pregenomic RNA (pgRNA) in each cell using droplet digital polymerase chain reaction.

Results

The proportion of

SUBMITTER: Balagopal A 

PROVIDER: S-EPMC7137881 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature