Determining isoleucine side-chain rotamer-sampling in proteins from ¹³C chemical shift.

ABSTRACT: Chemical shifts are often the only nuclear magnetic resonance parameter that can be obtained for challenging macromolecular systems. Here we present a framework to derive the conformational sampling of isoleucine side chains from ¹³C chemical shifts and demonstrate that side-chain conformations in a low-populated folding intermediate can be determined.

SUBMITTER: Siemons L 

PROVIDER: S-EPMC7138115 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature