Uterine Patterning, Endometrial Gland Development, and Implantation Failure in Mice Exposed Neonatally to Genistein.

ABSTRACT:

Background

Embryo implantation relies on precise hormonal regulation, associated gene expression changes, and appropriate female reproductive tract tissue architecture. Female mice exposed neonatally to the phytoestrogen genistein (GEN) at doses similar to those in infants consuming soy-based infant formulas are infertile due in part to uterine implantation defects.

Objectives

Our goal was to determine the mechanisms by which neonatal GEN exposure causes implantation defects.

Methods

Female mice were exposed to GEN on postnatal days (PND)1-5 and uterine tissues collected on PND5, PND22-26, and during pregnancy. Analysis of tissue weights, morphology, and gene expression was performed using standard histology, confocal imaging with three-dimensional analysis, real-time

SUBMITTER: Jefferson WN 

PROVIDER: S-EPMC7138129 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature