Glutamine supplementation improves the efficacy of miltefosine treatment for visceral leishmaniasis.

ABSTRACT:

Background

The disturbance of host metabolic pathways by Leishmania parasites has crucial consequences for the activation status of immune cells and the outcome of infection. Glutamine has been described as an immunomodulatory amino acid, yet its role during Leishmania infection is still unknown.

Methods

We performed transcriptomics in uninfected and L. donovani-infected macrophages 6 hours post-infection. Glutamine quantification by HPLC was assessed in the supernatant of macrophages throughout the infection course. For experimental L. donovani infections, mice were infected with 1.0 x 108 stationary L. donovani promastigotes. Glutaminase (GLS) chemical inhibition was performed using BPTES and glutamine was administered throughout infection. For combined therapy experiment,

SUBMITTER: Ferreira C 

PROVIDER: S-EPMC7138311 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature