EXP1 is required for organisation of EXP2 in the intraerythrocytic malaria parasite vacuole.

ABSTRACT: Intraerythrocytic malaria parasites reside within a parasitophorous vacuole membrane (PVM) that closely overlays the parasite plasma membrane. Although the PVM is the site of several transport activities essential to parasite survival, the basis for organisation of this membrane system is unknown. Here, we performed proximity labeling at the PVM with BioID2, which highlighted a group of single-pass integral membrane proteins that constitute a major component of the PVM proteome but whose function remains unclear. We investigated EXP1, the longest known member of this group, by adapting a CRISPR/Cpf1 genome editing system to install the TetR-DOZI-aptamers system for conditional translational control. Importantly, although EXP1 was required for intraerythrocytic development, a previously rep

SUBMITTER: Nessel T 

PROVIDER: S-EPMC7138706 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature