MicroRNA-429/Cxcl1 Axis Protective Against Oxygen Glucose Deprivation/Reoxygenation-Induced Injury in Brain Microvascular Endothelial Cells.

ABSTRACT:

Objective

The objective of the present work was to study the role of Cxcl1 in cerebral ischemia-reperfusion (I/R) injury and to in-depth explore its pathogenesis.

Methods

The expression of Cxcl1 based on the public data was analyzed. Then, we constructed an oxygen glucose deprivation/reoxygenation (OGD/R) model in vitro using mice brain microvascular endothelial cells (BMECs) to simulate cerebral I/R in vivo.

Results

The results of quantitative real-time polymerase chain reaction assay uncovered that Cxcl1 showed higher expression while miR-429 showed lower expression in BMECs damaged by OGD/R, whereas overexpression of Cxcl1 or inhibition of miR-429 expression can strengthen this effect. Hereafter, through dual luciferase reporter assay, we verified that miR-429 dire

SUBMITTER: Leng J 

PROVIDER: S-EPMC7139192 | biostudies-literature | 2020 Apr-Jun

REPOSITORIES: biostudies-literature