Insights into Interactions of Flavanones with Target Human Respiratory Syncytial Virus M_2-1 Protein from STD-NMR, Fluorescence Spectroscopy, and Computational Simulations.

ABSTRACT: The human Respiratory Syncytial Virus (hRSV) is the most frequent agent of respiratory infections in infants and children with no currently approved vaccine. The M_2-1 protein is an important transcriptional antitermination factor and a potential target for viral replication inhibitor development. Hesperetin (HST) and hesperidin (HSD) are flavonoids from the flavanone group, naturally found in citrus and have, as one of their properties, antiviral activity. The present study reports on the interactions between hRSV M_2-1 and these flavanones using experimental techniques in association with computational tools. STD-NMR results showed that HST and HSD bind to M_2-1 by positioning their aromatic rings into the target protein binding site. Fluorescence quenching

SUBMITTER: Piva HMR 

PROVIDER: S-EPMC7139479 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature